Calli tissue induction for Camellia nitidissima anther filaments and its sensitivity to antibiotics

This study established calli tissue induction system of Camellia nitidissima through In vitro culture from tender anther filaments of immature buds. The results showed that the suitable explant disinfection was soaked in 70% ethyl alcohol for 10 min. The optimal mediums for the callus induction and its multiplication was Murashige and Skoog（ MS） medium supplemented with 0.5 mg/L N-phenyl-N’-1，2，3-thiadiazol-5-ylurea（ TDZ），1.0 mg/L 2，4-Dichlorophenoxyacetic acid（ 2，4-D），and 30 g/L sucrose（ pH 5.8） in the dark at 25（±1）℃. And the influences of kanamycin and hygromycin on calli induction and multiplication were studied. The results showed that lethal doses of kanamycin and hygromycin to anther filament explants were 100 mg/L and 30 mg/L，respectively； either 75 mg/L kanamycin or 20 mg/L hygromycin could inhibit the calli production from the explant of anther filaments；either 100 mg/L kanamycin or 25 mg/L hygromycin could inhibit proliferiation of calli.